Journal: Materials Today Bio
Article Title: Microalgal-enhanced cerium oxide nanotherapeutics for alleviating inflammatory bowel disease via scavenging reactive oxygen species and modulating gut microbiota in colitis
doi: 10.1016/j.mtbio.2025.101945
Figure Lengend Snippet: SP@CSC alleviate IBD Inducing Factors in Macrophages. Cytoprotective effect of different treatments against H 2 O 2 -induced oxidative stress in RAW264.7 cells (a) and HT-29 cells (b). (c) Flow cytometry analysis of DCFH-DA staining in LPS-induced (1 μg/mL) RAW264.7 cells under various treatment conditions, along with (d) the corresponding quantitative evaluation. (e) Flow cytometry analysis of the proportions of CD86-positive and CD206-positive macrophages after LPS stimulation for 24 h. (f) Quantification of CD86-positive and CD206-negative cells across all groups. ELISA assays of typical proinflammatory of TNF-α (g), IL-6 (h). (i) Western blot assay for Nrf2, and HO-1expression of Raw 264.7 cells after LPS stimulation and treated with different treatments. Data are presented as mean ± S.D (n = 3 per group). Statistical significance was assessed using one-way ANOVA test. ∗ P < 0.05, ∗∗ P < 0.01.
Article Snippet: Antibodies for flow cytometry, specifically CD206-PE and CD86-FITC, along with ELISA kits for catalase (CAT), superoxide dismutase (SOD), interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α), and interleukin-17 (IL-17), were purchased from Elabscience Biotechnology Co., Ltd. (Wuhan, China).
Techniques: Flow Cytometry, Staining, Enzyme-linked Immunosorbent Assay, Western Blot